The aim of present study is to prepare and evaluate HPMC AS enteric coated microspheres to avail the drug at intestine and its distal part using Budesonide as model drug. The microspheres were prepared by emulsion solvent-evaporation method using HPMC AS MF. A 32 full factorial design was applied to optimize the formulation variables. From the preliminary study, it was concluded that independent variables Span 80 concentration (X1) and Polymer: Drug ratio (X2) showed significant effect on the dependable responses. All prepared microsphere were free flowing and spherical in shape. Optimized batch exhibit microspheres size 74.49 �µm, encapsulation efficiency 62.09 %w/w, Drug Release2hr 5.32 % and Drug Release5hr 81.3 %. Results of 32 full factorial design confirmed that independent variables has significant effect over the dependent responses which can be seen from the surface response graphs. The linear correlation plots drawn between the predicted and the observed responses demonstrated higher values of R2 (ranging between 0.991 and 0.999), indicating excellent fitting of the model. Upon comparison of the observed responses with that of the predicted responses, the standard error varied between 0.2250 and 2.35. Thus, the low magnitudes of standard error as well as the significant values of R2 in the current study indicate a high prognostic ability of BDS-HPMC AS MF microsphere optimization using 32 full factorial design.
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